Ratiometric imaging
Ratiometric imaging requires rapid wavelength switching and separating out the signals
for each wavelength. For these fast wavelength changes the Agilent Polychrome 5000 has been designed.
Traditionally a rotary filter changer has been used to change wavelength, but now the Agilent
Polychrome 5000 can do wavelength changes in less than 2 ms without causing any vibrations.
In addition an external board, independent from the PC ensures exact timing of illumination
and imaging in μs accuracy (Imaging System).
Ratio imaging can also be performed with a ratiomentric dye pair composed of two different
calcium-sensitive dyes, such as Fura-2 and Fura red.
In addtion Calcium-sensitive dyes may be combined with a calcium-insensitive marker, such
as Calcium Green and Texas Red (GFP-applications).
