
•	Introduction
•	Calcium imaging and imaging of other ions
•	Selection of Fluorescent dyes
•	Ratiometric imaging
•	Combining Imaging with electrophysiology or other equipment
•	Techniques and approaches in high speed calcium imaging
•	Equipment for highest speed Imaging
•	References

Hotline

Information downloads

Application note: Calcium imaging (217 KB)

Calcium Imaging

References:

Kullmann P.H.M., Ene F.A., Kandler K. 2002.Glycinergic and GABAergic calcium response in the developing lateral superior olive. European Journal of Neuroscience 15:1093-1104
Ene F.A., Kullmann P.H.M., Gillespie D.C., Kandler K. 2003. Glutamatergic calcium response in the developing lateral superior olive: Receptor types and their specific activation by synaptic activity patterns. J Neurophysiol. 90:2581-2591
Ellis-Davis G.C.R., Kaplan J.H., Barsotti R.J. 1996. Laser photolysis of caged calcium: Rates of calcium release ba nitrophenyl-EGTA and DM-Nitrophen. Biophys.J. 70:1006-1016. (properties of caged compounds)
Escobar A.L., Cifuentes F., Vergara J.L. 1995. Detection of Ca²⁺ transient elicited by flash photolysis of DM-Nitrophen with fast calcium indicator. FEBS Letters 364:335-338. (porperties of caged compounds)
Helmchen F., Imoto K., Sakmann B. 1996. Ca²⁺ buffering and action potential-evoked Ca²⁺ signalling in dendrites of pyramidal neurons. Biophys.J. 70:1069-1081. (Fluorescence measurements from nerve cells: theoretical and practical aspects to Ca²⁺ buffer capacities)
Kötter R., Staiger J.F., Zilles K., Luhmann H.J. 1998. Analysing functional connectivity in brain slices by a combination of infrader video microscopy, flash photolysis of caged compounds and scanning methods. Neurosc. Letter 86:265-277 (UV-Flash/flash photolysis of caged glutamate)
Naraghi M. 1997. T-jump study of calcium binding kinetics of calcium chelators. Cell Calcium. (binding kinetics of calcium chelators)
Neher E. 1995. The use of fura-2 for estimating Ca buffers and Ca fluxes. Neurophyrmacology 34:1423-1442 (theroretical and practical aspecs to photometry and fluorescence decrements)
Rettig J., Heinemann C., Ashery U., Sheng Z-H., Yokoyama C.T., Catterall W.A., Neher E. 1997. Alteration of Ca²⁺ dependence of neurotransmitter release ba disruption of Ca²⁺ Channel/Syntaxin Interaction. J.Neurosc. 17:6647-56 (ViewFinder/Photometry: Fluorescence Measurements from nerve terminals)
Xu T., Naraghi M., Kang H., Neher E. 1997. Kinetic suties of Ca²⁺ binding and Ca²⁺ clearence in the cytosol of adrenal chromaffin cells. Biophys.J. 73:532-545. (Polychrome: photolysis, Ca²⁺ measurement)