
•	Introduction
•	The point spread function
•	Deconvolution by adaptive inverse modelling - the TILLvisDECO approach
•	Requirements for optimal processing of image data in deconvolution experiments
•	Equipment needed for high quality deconvolution results
•	References

Hotline

Deconvolution and TILLvisDECO package

References

Sapia, M., I. Greenshields, M. Fox, L. M. Loew, J. Schaff, and G. Coutu. (1997). Three-dimensional deconvolution of optical microscope images using adaptive inverse modelling. Proceedings of the 40th IEEE Midwest Conference on Circuits and Systems, Sacramento, CA.
Loew, L.M., R.A. Tuft, W. Carrington and F.S. Fay (1993). Imaging in 5 dimensions: Time dependent membrane potentials in individual mitochondria. Biophys. J. 65:2396-2407
Les Loew's lab homepage (Centre for Biomedical Imaging Technology (CBIT) at the University of Connecticut Health Centre)

See also here for more information on Inverse filters.

General Interest: Peter J. Shaw, In "Handbook of Biological Confocal Microscopy", edited by James B. Pawley, 2nd edition ISBN 0-306-44826-2, (See chapter 23).
The molecular expressions web site contains a number of articles on the topic of deconvolution in optical microscopy