Fluorescence resonance energy transfer (FRET) microscopy
FRET is an interaction of two fluorochromes depending on the distance of these two fluorochromes.
One fluorochrome (the Donor) transfers the excitation energy directly to a second fluorochrome (the Acceptor) without the emission of a photon. Typically two fluorochromes are used that have overlapping emission and excitation wavelengths but FRET can be achieved in several ways. The two interacting fluorochromes need to be close together so that FRET takes place;
roughly speaking in the range of a few nanometers. In a FRET experiment this interaction of the two fluorochromes needs to be detected. For a more detailed introduction read the TILL application note or see here.
TILL Photonics offers several solutions for FRET measurements which have specific advantages:
|For motorized switching between up to 3 different FRET pairs||⇒||Use the iMIC dichrotome|
|For ultimate image resolution||⇒||Use the iMIC dual camera module as part of iMIC 42|
|For fastest FRET measurements or FRET signals that need to be synchronized with electrophysiologicaly derived signals||⇒||Use the TILL dual emission photometry system|