GFP - Imaging Fluorescent Proteins

References & links

Links:

Most commercially available XFPs as cloning vectors, antibodies against XFPs, vectors for RCPs, and more:

BD-Bioscience (formerly Clontech)

Fluorescent filters for your living color application:
Chroma
AHF

Antibodies against GFP:
Molecular Probes

References:

• Ormo M, Cubitt AB, Kallio K, et al.: Crystal structure of the Aequorea victoria green fluorescent protein. Science 273, 1392-1395 (1996)
• Sawano A & Miyawaki A: Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis. Nucleic Acids Res. 28, E78 (2000)
• Griesbeck O, Baird GS, Campbell RE, Zacharias DA & Tsien RY: Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. J. Biol. Chem. 276, 29188-29194 (2001)
• Nagai T et al.: A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nature Biotechnol. 20, 87-90 (2002)
• Campbell RE et al.: A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. USA 99, 7877-7882 (2002)
• Patterson, GH & Lippincott-Schwartz, J: A photoactivatable GFP for selective photolabeling of proteins and cells. Science 297, 1873-1877 (2002)
• Ando R, Hama H, Yamamoto-Hino M, Mizuno H & Miyawaki A: An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Procl. Natl. Acad. Sci. USA 99, 12651-12656 (2002)
• Waterman-Storer CM, Desai A, Bulinski JC & Salmon ED: Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cells. Curr. Biol. 8 (22), 1227-1230 (1998)
• Servant G et al.: Polarization of chemoattractant receptor signaling during neutrophil chemotaxis. Science 287, 1037-1040 (2000)
• Meili R et al.: Chemoattractant-mediated transient activation and membrane localization of Akt/PKB is required for efficient chemotaxis to cAMP in Dictyostelium. EMBO J. 18 (8), 2092-2105 (1999)
• Oancea E & Meyer T: Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals. Cell 95, 307-318 (1998)
• Kaether C & Gerdes HH: Visualization of protein transport along the secretory pathway using green fluorescent protein. FEBS Lett. 369, 267-271 (1995)
• Rudolf R, Salm T, Rustom A & Gerdes HH: Dynamics of immature secretory granules. Mol. Biol. Cell 12 (12), 1353-1365 (2001)
• Kneen M, Farinas J, Li Y & Verkman AS: Green fluorescent protein as a noninvasive intracellular pH indicator. Biophys. J. 74, 1591-1599 (1998)
• Miesenböck G, De Angelis DA & Rothman JE: Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394, 192-195 (1998)
• Jayaraman S, Haggie P, Wachter RM, Remington SJ & Verkman AS: Mechanism and cellular applications of a green fluorescent protein-based halide sensor. J. Biol. Chem. 275, 6047-6050 (2000)
• Rapizzi E et al.: Recombinant expression of the voltage-dependent anion channel enhances the transfer of Ca2+ microdomains to mitochondria. J. Cell Biol. 195 (4), 613-624 (2002)
• Nagai T, Sawano A, Park E & Miyawaki A: Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. USA 98, 3197-3202 (2001)