| • | Introduction |
| • | Application |
| • | What happens at the water/glass interface? |
| • | Technical realization |
| • | TIRF solutions |
| • | Advantages of the Agilent TIRF |
Hotline  |
TIRF - Total Internal Reflection Fluorescence
Applications
- Tracking of secretory granules in intact cells
- Selective visualization of cell/substrate contact regions
- Protein translocation
- Selective detection of membrane proteins, e.g. ion channels
- Visualization of single molecules near the surface
- Force measurements on single molecular contacts
- Long term fluorescence observation of living cells