Laser Line Combiner

TILL's Laser Line Combiner is an easy solution for a common demand in fluorescence microscopy: The combination of different laser lines in the same experiment. This allows the detection of multiple dye combinations using laser-based methods such as TIRF, FRAP, photoactivation and uncaging.

The Laser Line Combiner combines the beams of up to four individual lasers by feeding them into a single fiber. An accusto-optic tunable filter (AOTF) allows to control gating and attenuating of every single laser either manually or via software.

After passing the AOTF the light from the lasers is coupled into a monomode fi ber. This fiber accommodates wavelengths from 400 to 650 nm and maintains the lasers’ original polarization.

An additional mechanical shutter that can be triggerd by TTL signals is added for laser safety reasons.

Enable experiments that require a sophisticated combination of methods using the TILL Laser Line Combiner.

Key benefits

• Flexibility for detecting a variety of different dye combinations in a specimen
• Broad spectrum of laser wavelengths (405 – 643 nm)
• Programming of user defined experiments through AOTF and software control
• Reduced photobleaching and phototoxicity
• Exact timing of illumination for image acquisition
• High stability and repeatability of measurements
• Higher image acquisition rates
• incorporation of additional laser lines
• Ease of use