Q: How do I set up a wavelength protocol?
A: For setting up an aquisition Protocol you will need the TILL Protocol Editor. The TILL Protocol Editor is an additional program which enables you to set up much more flexible protocols than with the Grab Settings/Image Series (Which is also not supported any more!). You can run the TILL Protocol Editor from TILLvisION or as an independent program.
The structure of the protocols which are arranged in the so-called Experiment Plan is very similar to the tree structure of the Windows NT Explorer. To edit the protocol you can use the Experiment menu or the appropriate Append and Child buttons. Full Drag & Drop and Copy & Paste support is provided too.
The screenshot shows a more or less typical protocol. It acquires a "dark" image at experiment start (you may edit the object names in the experiment plan to work with consistently named image objects). Then it performs 100 ratios synchronized with an external trigger event. Each 340 nm Fluorescence Image Exposure Time is reported via a TTL Pulse. After the ratio processing a final dark image is acquired.
The experiment setup is simple. You only have to put some objects in the experiment plan (or load a provided setup from file). By selecting the object in the tree view, the respective options page is shown on the right-hand side.
The TILL Protocol Editor knows the physical capabilities of the supported hardware - therefore, while editing the protocol you are constantly informed about your experiment timing.
Before each protocol execution the TILL Protocol Editor checks your system resources. If you have got enough physical memory to keep the high speed images, or if your timing requirements could be fulfilled by your hard disk speed - everything works fine.
If your system resources are too low to meet your experiment requirements, you will get the option to either acquire as fast as possible or to re-edit your protocol.
If your system resources are too low to meet your experiment requirements, you will get the option to either acquire as fast as possible or to re-edit your protocol.