FAQ for TILLvisION

How to start and benchmark a protocol?

The experiment setup is stored in a so-called Till Protocol. You can save it as single file and / or embed it into a TILLvisION Workspace (*.vws; visION document).

To execute an embedded protocol you simply have to select it in the workspace view ...

... and click on the Execute Protocol button.

Experiment Check Phase

Before executing a protocol, the Imaging System has to check if your system meets the experiment requirements like hard disk space, hard disk and memory speed, etc. The system performance may vary due to memory segmentation and parallel running processes.

While benchmarking the system, the TILL Protocol Editor first checks if the acquisition timing is "slow" enough to write directly to hard disk (about 2-3 MB/sec). If not, TILLvisION has to allocate physical memory exclusively for storing images during acquisition. You can approximate your physical memory needs by calculating the size of all images to acquire high speed and continuity. Add approx. 24 MB for Windows NT and the TILLvisION application.

The benchmarking is done on a per image sequence base, so it is possible to set up a protocol with high speed images at experiment start (written to memory) followed by less fast image acquistion (written to disk) and high speed acquisition at the end (again kept in memory).

Experiment Acquisition Phase

If your data acquisition is not too fast for user interaction, you can mark events while acquiring data. This may be useful if you want to know later at what time in relation to image acquisition you have applied your specific chemical.

At the end of the experiment TILLvisION writes all images to disk to free physical memory for further experiments. The offline handling of experiment data makes use of the unique Big Image Driver, which transparently handles images on disk as if they were in memory. This feature allows the fast handling of images or image sequences much bigger than physical memory. Normally the location of images (memory or disk) is specified automatically.